M042030

Biotin Phosphoramidite

configured for ABI

• 동의어(들): Biotin amidite
• 실험식(Hill 표기법): C₄₆H₆₄N₅O₈PS
• CAS Number: 1217500-22-5
• Molecular Weight: 878.07
• MDL number: MFCD00676535
• UNSPSC 코드: 41116105
• PubChem Substance ID: 329817780
• NACRES: NA.21

속성

• 제품 라인: Proligo Reagents
• 분석: ≥96% (³¹P-NMR)
• 적합성: conforms to structure for H-NMR
• 호환성: configured for ABI
• 저장 온도: −20°C
• SMILES string: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
• InChI key: WSDQYLGSIUJJRU-ZMKGMYJVSA-N

설명

일반 설명

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for ABI^™ Synthesizers

법적 정보

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable