M042080

Biotin Phosphoramidite

configured for PerkinElmer, configured for Polygen

• 동의어(들): Biotin amidite
• 실험식(Hill 표기법): C₄₆H₆₄N₅O₈PS
• CAS Number: 1217500-22-5
• Molecular Weight: 878.07
• MDL number: MFCD00676535
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329817781
• NACRES: NA.21

속성

• 제품 라인: Proligo Reagents
• Quality Level: 200
• 분석: ≥96% (³¹P-NMR)
• 적합성: conforms to structure for H-NMR
• 호환성: configured for PerkinElmer; configured for Polygen
• 저장 온도: −20°C
• SMILES string: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
• InChI key: WSDQYLGSIUJJRU-ZMKGMYJVSA-N

설명

일반 설명

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis.

The photocleavable biotin phosphoramidite reagent is compatible with automated DNA/RNA synthesizers using phosphoramidite chemistry. The length of the p-aminophenethyl spacer group allows streptavidin affinity purification of the oligonucleotide by reverse-phase HPLC. Biotinlabeling is compatible with PCR and most hybridization techniques.Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for Expedite^™ and PolyGen^® Synthesizers.

애플리케이션

Biotin Phosphoramidite is suitable for the synthesis of 5′-biotinylated oligonucleotides whose applications are seen in ligase-mediated gene detection, direct dideoxy sequencing following PCR, and non-radioactive sequencing of DNA.

법적 정보

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

PolyGen is a registered trademark of PolyGen GmbH.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable