LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• 동의어(들): Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• 효소 위원회 번호: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Achromobacter lyticus
• Quality Level: 200
• 재조합: expressed in E. coli (206-473aa)
• Grade: Proteomics Grade
• 양식: ready-to-use solution
• 농도: 1 mg/mL
• 최적 pH: 8.5
• pH 범위: 9.0-9.5(catalytic)
• UniProt 수납 번호: P15636
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

특이성

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

애플리케이션

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

포장

1mg/ml in Plastic

제조 메모

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

저장 및 안정성

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

기타 정보

For R&D only.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable