추천 제품
생물학적 소스
goat
Quality Level
결합
unconjugated
항체 형태
IgG fraction of antiserum
항체 생산 유형
primary antibodies
클론
polyclonal
양식
lyophilized powder
종 반응성
mouse
기술
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable
UniProt 수납 번호
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... LIF(3976)
mouse ... Lif(16878)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
특이성
The antibody neutralizes the biological activity of recombinant mouse LIF and also neutralizes the biologicl activity of recombinant human LIF at a 10-fold higher IgG concentration. Based on ELISA and immunoblotting, this antibody shows <10% cross-reactiity with recombinant human LIF.
면역원
purified, E. coli-derived, recombinant mouse LIF.
애플리케이션
For Western blot a concentration of 0.1 μg/ml is recommended. For immunohistochemistry, a working dilution of 5-15 μg/ml is recommended. The neutralization dose is typically 80-480 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.
Optimal dilutions should be determined by the end user.
Optimal dilutions should be determined by the end user.
생화학적/생리학적 작용
Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.
물리적 형태
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.
제조 메모
Protein A or G purified
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
L Q Cai et al.
Cytokine, 12(11), 1676-1682 (2000-10-29)
Leukaemia inhibitory factor (LIF) is a pleiotrophic cytokine. Recent reports indicate that LIF is relevant to murine embryo implantation. In this work, results of indirect immunofluorescence under a confocal microscope illustrated that LIF was mainly located in the uterine lumen
Rozanne Arulanandam et al.
Experimental cell research, 316(5), 875-886 (2009-10-27)
Rac1 (Rac) is a member of the Rho family of small GTPases which controls cell migration by regulating the organization of actin filaments. Previous results suggested that mutationally activated forms of the Rho GTPases can activate the Signal Transducer and
Sylvian Bauer et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(46), 12089-12099 (2006-11-17)
Although neural stem cells (NSCs) persist in various areas of the adult brain, their contribution to brain repair after injury is very limited. Treatment with exogenous growth factors can mitigate this limitation, suggesting that the brain environment is normally deficient
L C Hunt et al.
Experimental cell research, 316(6), 1002-1009 (2009-12-08)
Leukemia inhibitory factor (LIF) is an important regulator of skeletal muscle regeneration and has been suggested to be mitogenic for myogenic cells because it has been shown to increase the quantity of myoblast cells grown in culture over extended periods
Tsuyoshi Tsukada et al.
PloS one, 10(6), e0129011-e0129011 (2015-06-05)
Recent studies in rodents suggest that maternal immune activation (MIA) by viral infection is associated with schizophrenia and autism in offspring. Although maternal IL-6 is though t to be a possible mediator relating MIA induced these neuropsychiatric disorders, the mechanism
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.