L6533

Lectin from Dolichos biflorus (horse gram)

biotin conjugate, buffered aqueous solution

• 동의어(들): Dolichos biflorus agglutinin, DBA
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 결합: biotin conjugate
• Quality Level: 200
• 형태: buffered aqueous solution
• 구성: Protein, ≥2.5 mg/mL biuret
• 라벨링 범위: ≥3 mol biotin per mol lectin
• 저장 온도: −20°C

설명

일반 설명

Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.

애플리케이션

Lectin from Dolichos biflorus (horse gram) has been used:

• to characterize mouse uterine natural killer (uNK) cells
• in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
• as a species-specific gonocyte marker for the determination of donor cell colonization
• to label undifferentiated spermatogonia/cat testicular tissue

생화학적/생리학적 작용

Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.

DBA has anti-A₁ human blood group specificity, useful for distinguishing between A₁ and A₂ blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

포장

Sold on the basis of mg active conjugate.

물리적 형태

contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca²⁺

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable