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Merck
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Key Documents

L4417

Sigma-Aldrich

Anti-phospho-Lck (pTyr192) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.44

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous glycerol solution

종 반응성

human

기술

western blot: 1:1,000 using full length recombinant human Lck protein.

배송 상태

wet ice

저장 온도

−20°C

타겟 번역 후 변형

phosphorylation (pTyr192)

유전자 정보

human ... LCK(3932)
mouse ... Lck(16818)

일반 설명

Lck, also referred to as T cell-specific protein tyrosine kinase, is a 56kD protein that belongs to Src family of non-receptor tyrosine protein kinases and is expressed mainly in T cells. It has a pivotal role in the initiation of pre-TCR and T-cell antigen receptor (TCR)-linked signal transduction pathways. Anti-phospho-Lck (pTyr192) antibody reacts specifically with Lck (pTyr192) in humans.

면역원

synthetic phosphopeptide derived from the region of Lck that is phosphorylated on tyrosine 192. Human and mouse have 100% homology.

애플리케이션

Anti-phospho-Lck (pTyr192) antibody can be used in western blotting (diluted 1:1000) using full length recombinant human Lck protein.

물리적 형태

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide.

제조 메모

The antibody is preadsorbed to remove any reactivity towards a non-phosphorylated Lck.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

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문서 라이브러리 방문

Hong Wang et al.
Cellular signalling, 23(1), 249-258 (2010-09-21)
Pre-T-cell receptor (pre-TCR) and TCR signals govern the development of T-lymphocytes. RhoH, a hematopoietic-specific and GTPase-deficient member of the RhoGTPase family, is required in the development of T-lymphocytes. Here we found that RhoH binds and modulates LCK, the non-receptor tyrosine

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