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Merck
모든 사진(3)

Key Documents

L4268

Sigma-Aldrich

Lipopolysaccharides from Klebsiella pneumoniae

purified by phenol extraction

동의어(들):

LPS

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About This Item

MDL number:
UNSPSC 코드:
12352201
NACRES:
NA.25

생물학적 소스

bacterial (Klebsiella pneumoniae)

Quality Level

형태

lyophilized powder

정제법

phenol extraction

불순물

<3% Protein (Lowry)

색상

white to faint yellow

solubility

water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

This product is phenol extracted from Klebsiella pneumoniae. The source strain is ATCC 15380.

생화학적/생리학적 작용

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

제조 메모

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

기타 정보

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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Biosensors & bioelectronics, 99, 312-317 (2017-08-09)
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Christian Maueröder et al.
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