L2895

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B₄ (BSI-B₄), FITC conjugate, lyophilized powder

• 동의어(들): Bandeirea simplicifolia agglutinin, BS-I
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 결합: FITC conjugate
• Quality Level: 200
• 형태: lyophilized powder
• 효능: <50 μg per mL agglutination activity (using human blood group B erythrocytes)
• 구성: Protein, ≥70% Lowry (balance sodium citrate)
• 라벨링 범위: ≥1 mol FITC per mol lectin
• 저장 온도: 2-8°C

설명

일반 설명

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

애플리케이션

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for microglia detection in murine astrocytes culture by staining the culture with lectin BS1-B4. It has also been used in the preparation of staining solution to incubate the tissue sections.

생화학적/생리학적 작용

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling, because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

포장

Package size based on protein content by Lowry.

기타 정보

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B₄, BSI-AB₃, BSI-A₂B₂, BSI-A₃B and BSI-A₄. BSI-B₄ is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A₄ has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

분석 메모

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)