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Merck
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Key Documents

L2262

Sigma-Aldrich

Lipopolysaccharides from Salmonella enterica serotype typhimurium

purified by gel-filtration chromatography

동의어(들):

LPS

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About This Item

MDL number:
UNSPSC 코드:
12352201
NACRES:
NA.25

생물학적 소스

Salmonella enterica (Serotype typhimurium)

Quality Level

형태

lyophilized powder

정제법

gel-filtration chromatography

불순물

<1% Protein

색상

white to faint yellow

solubility

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

This product is extracted from Salmonella typhimurium and purified by gel filtration. The source strain is ATCC 7823.

애플리케이션

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

생화학적/생리학적 작용

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

제조 메모

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

기타 정보

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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