콘텐츠로 건너뛰기
Merck
모든 사진(6)

Key Documents

H9286

Sigma-Aldrich

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-H3K9ac

로그인조직 및 계약 가격 보기


About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

분자량

antigen ~17 kDa

종 반응성

bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken

농도

2-2.5 mg/mL

기술

indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
microarray: suitable
western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

acetylation (Lys9)

일반 설명

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:
  • indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
  • microarray
  • western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

면역원

synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

애플리케이션

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:
  • indirect immunofluorescence
  • immunoblotting
  • immunohistochemistry

생화학적/생리학적 작용

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.
In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

이미 열람한 고객

Slide 1 of 1

1 of 1

P66shc Negatively Regulates Insulin-like Growth Factor I Signal Transduction Via Inhibition Of P52shc Binding To Src Homology 2 Domain-containing Protein Tyrosine Phosphatase Substrate-1 Leading To Impaired Growth Factor Receptor-bound Protein-2 Membrane Recruitment.
Xi, G., et al.
Molecular Cancer Therapeutics, 22, 2162-2175 (2008)
Genliang Li et al.
Cytotechnology, 69(1), 75-87 (2016-11-30)
As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding
Gang Xi et al.
Molecular endocrinology (Baltimore, Md.), 22(9), 2162-2175 (2008-07-09)
Our previous studies have indicated an essential role of p52shc in mediating IGF-I activation of MAPK in smooth muscle cells (SMC). However, the role of the p66 isoform of shc in IGF-I signal transduction is unclear. In the current study
Social isolation-induced epigenetic changes in midbrain of adult mice
Siuda D, et al.
Journal of Physiology And Pharmacology, 65(2), 247-255 (2014)
Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome.
R D Kornberg et al.
Cell, 98(3), 285-294 (1999-08-24)

관련 콘텐츠

Chromatin structure plays a fundamental role in regulating processes that take place on the DNA, such as transcription, replication, and recombination, all of which occur on nucleosomal templates. Therefore, mapping the position of histones selectively modified and histone variants or non-histone components of chromatin in specific DNA sequences, can provide valuable insights into how these proteins (and their modifications) function in a chromatin context.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.