추천 제품
Grade
for molecular biology
Quality Level
설명
recommended for cultivation of recombinant E. coli.
무균
non-sterile
형태
powder
구성
KCl, 0.186 g/L
MgSO4, 2.4 g/L
NaCl, 0.5 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L
기술
microbiological culture: suitable
pH
6.8-7.2(2.8% solution)
응용 분야
food and beverages
microbiology
저장 온도
room temp
적합성
nonselective for Escherichia coli
nonselective for coliforms
일반 설명
Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.
애플리케이션
Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
특징 및 장점
Hannahan′s Broth (SOB) powder provides:
- Standard formulation
- An inexpensive alternative to liquid
재구성
Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
기타 정보
Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.
관련 제품
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Difco Manual, 444-444 (1998)
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
Biotechnology for Biofuels, 12, 150-150 (2019)
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth
Nature protocols, 11(8), 1554-1571 (2016-07-29)
The thermostability of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals that are suitable for structure determination. However, many mammalian MPs are too unstable for crystallization. We developed
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