H7006
HEPES sodium salt
≥99.0% (titration)
동의어(들):
Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
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모든 사진(5)
About This Item
실험식(Hill 표기법):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
EC Number:
MDL number:
UNSPSC 코드:
12161700
PubChem Substance ID:
NACRES:
NA.25
추천 제품
Quality Level
분석
≥99.0% (titration)
형태
powder
저장 조건
dry at room temperature
기술
HPLC: suitable
색상
white
pH
10-12 (1 M in water)
유용한 pH 범위
6.8-8.2
pKa(25 °C)
7.5
solubility
water: 0.1 M, clear, colorless
적합성
suitable for mass spectrometry
응용 분야
diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics
저장 온도
room temp
SMILES string
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
애플리케이션
HEPES buffer finds application in:
It has been used:
- Most studies with metal ions
- Substitute for Tris and phosphate in studies with metal ions
- Grinding buffer in plant studies
- Running buffer in gel electrophoresis
- Buffer in electroporation studies
- Bradford or bicinchoninic acid (BCA) assays
- Extraction buffer to prevent damage of proteins in red blood cells
It has been used:
- as a buffer component in the preparation of a low sucrose buffer
- as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions
특징 및 장점
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Highly soluble in water
- Negligible metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
물리적 형태
Lyophilized, spray dried, or crystallized material.
기타 정보
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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