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Merck
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Key Documents

추가 자료

H3537

Sigma-Aldrich

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

동의어(들):

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

CAS Number:
7365-45-9
MDL number:
MFCD00006158
UNSPSC 코드:
12161700
PubChem Substance ID:
329815199
NACRES:
NA.25

product name

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Grade

BioPerformance Certified

Quality Level

200

무균

0.2 μm filtered

형태

liquid

농도

1 M

기술

cell culture | mammalian: suitable

불순물

Bioburden, tested
 DNase, RNase, Protease, Nickase, free
 endotoxin, tested

pH

5.0-6.0

유용한 pH 범위

6.8-8.2

양이온 미량물

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

적합성

suitable for molecular biology

응용 분야

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

애플리케이션

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
  • To recover purified ribonucleotide
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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