H2662

Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-HD7, Anti-HD7A, Anti-HDAC7A
• MDL number: MFCD04040364
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~105 kDa
• 종 반응성: mouse, human, rat
• 기술: immunoprecipitation (IP): 1.0-1.5 μg using RIPA extract of 293T cells expressing recombinant human HDAC7; indirect immunofluorescence: 1:50 using rat NRK cells; western blot: 1:1,000 using whole extracts of mouse NIH-3T3 cells
• UniProt 수납 번호: Q8WUI4
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HDAC7(51564) ; mouse ... Hdac7(56233) ; rat ... Hdac7a(84582)

설명

일반 설명

Mammalian histone deacetylases (HDACs) can be divided into three classes according to sequence homology. Class I consists of the yeast transcriptional regulatory protein Rpd3-like proteins HDAC1, HDAC2, HDAC3, and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Class III consists of the yeast sirtuin 2 (Sir2)-like proteins. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm.

특이성

Anti-Histone Deacetylase 7 recognizes human, mouse, and rat HDAC7.

면역원

Synthetic peptide corresponding to amino acids of human HDAC7, conjugated to KLH.

애플리케이션

Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit has been used in

• indirect immunofluorescene
• immunoblotting
• immunoprecipitation

생화학적/생리학적 작용

Class II histone deacetylases (HDACs) have been implicated in the regulation of muscle differentiation. Interaction of HDAC4, -5, and -7 with members of the myocyte enhancer factor-2 (Mef2) family of transcription factors represses their transcriptional activity and prevents myogenesis. Shuttling of HDAC7 between the cell nucleus and the cytoplasm is controlled by a mechanism involving calmodulin independent kinase I (CaMKI) and 14-3-3 proteins. The HDAC7 enzymatic activity depends on its interaction with the class I HDAC3, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (N-COR). HDAC7 also interacts with the transcriptional repressor B-cell lymphoma 6 (BCL-6).

물리적 형태

Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable