H0160

HIV-1 gag

≥95% (SDS-PAGE), recombinant, expressed in E. coli, Galactosidase-tagged

• 동의어(들): p24 HIV antigen
• MDL number: MFCD01635027
• UNSPSC 코드: 12352204
• NACRES: NA.83

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥95% (SDS-PAGE)
• 분자량: 39 kDa (plus 114 kDa β-Gal tag)
• 농도: 1 mg/mL
• UniProt 수납 번호: P04591
• 저장 온도: −20°C
• 유전자 정보: human immunodeficiency virus 1 ... gag(155030)

설명

일반 설명

Contains amino acid residues [77-436] of HIV-1 gag antigen.

The human immunodeficiency virus 1 (HIV−1) gag antigen is a recombinant 39 kDa protein encoded by the GAG gene of the human immunodeficiency virus (HIV) type I expressed in and isolated from Escherichia coli. It has a β−galactosidase tag (114 kDa) on the N−terminus. HIV-1 Gag exists as monomers or lower-order oligomers in the cytosol. At the plasma membrane, it forms higher-order multimers.

애플리케이션

HIV-1 gag has been used in the coculture with an autologous cluster of differentiation T (CD4⁺ T) to study the ability of monocyte-derived dendritic cells (MDC1) to induce human immunodeficiency virus 1 (HIV−1) latency reversal (LR).

생화학적/생리학적 작용

The human immunodeficiency virus 1 (HIV−1) gag (p24) antigen reacts strongly with HIV positive serum and may be used as a positive control for evaluating antibodies to HIV. At transcription sites, HIV-1 Gag creates ribonucleoprotein complexes with unspliced viral RNA. The HIV-1 Gag protein is accountable for packaging genomic RNA (gRNA) and the assembly of immature viral particles.

Positive control for HIV antibodies.

물리적 형태

Solution in 8 M urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable