추천 제품
포장
pkg of 1 kit
제조업체/상표
Cytiva RPN5785
색상
ECF substrate: Off-white/white to yellowish ECF substrate dilution buffer: Colorless
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
Alkaline phosphatase dephosphorylates ECF™ Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.
The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.
The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.
애플리케이션
Vistra ECF™ substrate has been used in Western blotting.
특징 및 장점
- A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
- For use in western blotting, dot, and slot blotting applications
- Sensitivity comparable to chemiluminescence
- The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
- Eliminates need for darkroom and film
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
ECF is a trademark of Cytiva
신호어
Danger
유해 및 위험 성명서
Storage Class Code
12 - Non Combustible Liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Anti-atherosclerotic effects of small molecular weight compounds enhancing eNOS expression and preventing eNOS uncoupling.
Wohlfart P, et al.
Journal of Pharmacology and Experimental Therapeutics (2007)
Cindy Gueguen et al.
PloS one, 9(2), e87367-e87367 (2014-03-04)
Poly(ADP-ribose)polymerase and sirtuin 1 are both NAD(+)-dependent enzymes. In vitro oxidative stress activates poly(ADP-ribose)polymerase, decreases NAD(+) level, sirtuin 1 activity and finally leads to cell death. Poly(ADP-ribose)polymerase hyperactivation contributes to cell death. In addition, poly(ADP-ribose)polymerase inhibition restores NAD(+) level and
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