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Merck
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주요 문서

GE29-0915-96

Sigma-Aldrich

Superose® 6 Increase 10/300 GL

Cytiva 29-0915-96, 10/300 GL

동의어(들):

Superose® 6 Increase

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모든 사진(1)

About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

material

glass column
polyethylene filter

제품 라인

Superose®

분자량

5000-5,000,000 Da (globular proteins)

포장

package of 1 ea

제조업체/상표

Cytiva 29-0915-96

파라미터

<0.35 mL/min flow rate
<0.75 mL/min flow rate (20% ethanol at room temp)
<0.75 mL/min flow rate
<1.50 mL/min flow rate (water at room temp)
25-500 μL sample volume
3 Mpa

베드 용적

24 mL

Matrix

cross-linked agarose
polymeric

기질 활성군

agarose phase

cleaning

1-14

작동 범위

3-12

분리 기술

size exclusion (SEC)

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관련 카테고리

일반 설명

Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.

특징 및 장점

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

적합성

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.

저장 및 안정성

Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Storage Class Code

3 - Flammable liquids

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시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Anja Fux et al.
Cell chemical biology, 26(1), 48-59 (2018-11-13)
Detection of dynamic protein-protein interactions within complexes and networks remains a challenging task. Here, we show by the example of the proteolytic ClpXP complex the utility of combined chemical cross-linking and mass spectrometry (XL-MS) to map interactions within ClpP and

문서

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

프로토콜

Performing a Separation with Superose

Superose from GE Healthcare are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

관련 콘텐츠

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

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