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주요 문서

GE29-0510-21

HisTrap High Performance

Cytiva 29-0510-21, pack of 1 mL

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

포장

pack of 1 mL

제조업체/상표

Cytiva 29-0510-21

재고 정보

not available in North America

파라미터

flow rate
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

베드 크기

7 mm × 25 mm

베드 용적

1 mL

컬럼 I.D.

7 mm

Matrix

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-14(Ni2+-stripped medium.)

작동 범위

3-12(Ni2+-stripped medium)

용량

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.)
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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일반 설명

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

애플리케이션

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

특징 및 장점

  • High binding capacity, at least 40 mg/ml chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

법적 정보

HisTrap is a trademark of Cytiva

픽토그램

Environment

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

모두 보기

프로토콜

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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