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주요 문서

GE28-9893-33

Sigma-Aldrich

HiLoad® 16/600 Superdex® 75 pg

Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

동의어(들):

HiLoad 16/600

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About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

material

bed support (polyamide)
glass column
glass fiber (reinforced)

분자량

3000-70000 Da (globular proteins)

포장

pkg of 1 ea

제조업체/상표

Cytiva 28-9893-33

파라미터

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

베드 용적

120 mL

컬럼 길이 × I.D.

60 cm × 16 mm

Matrix

agarose-dextran composite
dextran-agarose particle platform
polymeric

기질 활성군

dextran-agarose phase
dextran-agarose phase

평균 부품 크기

34 μm

cleaning

1-14

작동 범위

3-12

분리 기술

size exclusion (SEC)

일반 설명

HiLoad® 16/600 Superdex® 75 prep grade are prepacked 120 mL XK columns designed for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

특징 및 장점

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

적합성

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

죄송합니다. 지금은 이 제품에 대한 COA이(가) 온라인에서 제공되지 않습니다.

도움이 필요하시면 연락하세요. 고객 지원 부서

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

프로토콜

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

관련 콘텐츠

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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