추천 제품
material
bed support (polyamide)
glass column
glass fiber (reinforced)
분자량
3000-70000 Da (globular proteins)
포장
pkg of 1 ea
제조업체/상표
Cytiva 28-9893-33
파라미터
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
베드 용적
120 mL
컬럼 길이 × I.D.
60 cm × 16 mm
Matrix
agarose-dextran composite
dextran-agarose particle platform
polymeric
기질 활성군
dextran-agarose phase
dextran-agarose phase
평균 부품 크기
34 μm
cleaning
1-14
작동 범위
3-12
분리 기술
size exclusion (SEC)
일반 설명
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
특징 및 장점
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
적합성
저장 및 안정성
분석 메모
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
프로토콜
This page shows how to refold proteins using gel filtration and products from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
관련 콘텐츠
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.