추천 제품
포장
pack of 10 × 1 mL
제조업체/상표
Cytiva 28-9852-55
저장 조건
(20% Ethanol)
베드 용적
1 mL
용량
~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
저장 온도
2-8°C
일반 설명
Protein G GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of Protein G Sepharose™ 4 Fast Flow.
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
애플리케이션
Fast and efficient manual purification of monoclonal and polyclonal antibodies
특징 및 장점
- Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
- Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
- High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
- High binding capacity such as Protein G GraviTrap™ ~ 20 mg human IgG/column
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
Daniel Neumeier et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(18), e2113766119-e2113766119 (2022-04-30)
The capacity of humoral B cell-mediated immunity to effectively respond to and protect against pathogenic infections is largely driven by the presence of a diverse repertoire of polyclonal antibodies in the serum, which are produced by plasma cells (PCs). Recent
문서
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
프로토콜
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare
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