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Key Documents

GE28-9670-66

Protein G Mag Sepharose Xtra

Cytiva 28-9670-66, pack of 2 × 1 mL

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

ligand

protein G

포장

pack of 2 × 1 mL

제조업체/상표

Cytiva 28-9670-66

저장 조건

(20% Ethanol, 10% medium slurry)

파라미터

Room temperature temp. range

기질

paramagnetic, spherical, highly cross-linked agarose particles

용량

27 mg binding capacity(human IgG/ml gel)

저장 온도

2-8°C

일반 설명

protein G Mag Sepharose Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG /mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

애플리케이션

Protein G Mag Sepharose Xtra magnetic beads combine proven affinity chromatography methods with the magnetic bead platform to improve simplification and ease-of-use in sample preparation. Mag Sepharose beads are dense, black and visible, and this makes them simple to handle. Less smearing effects and aggregate formations of the magnetic beads mean that no detergents are needed in the buffers.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.

특징 및 장점

  • Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
  • High purity and yield
  • Robust parallel screening of antibodies with high reproducibility
  • Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
  • Visible and dense Sepharose based magnetic beads give increased simplicity for handling
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

프로토콜

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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