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Key Documents

GE28-9365-51

Hisprep Fast Flow 16/10

Cytiva 28-9365-51

동의어(들):

16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column

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About This Item

UNSPSC 코드:
23151817
NACRES:
NA.56

포장

pkg of 1 ea

제조업체/상표

Cytiva 28-9365-51

파라미터

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

베드 크기

16 mm × 100 mm

베드 용적

20 mL

컬럼 I.D.

16 mm

기질

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

용량

~40 mg binding capacity(histidine-tagged protein)

적합성

suitable for bioprocess medium

관련 카테고리

일반 설명

HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

특징 및 장점

  • Optimized for convenient scale-up purification of histidine-tagged proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible leakage of Ni2+.
  • High binding capacity, approx. 40 mg/mL medium.
  • Convenient and time-saving 20 mL prepacked HiPrep format.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiPrep is a trademark of Cytiva
HisPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Environment

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

프로토콜

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.