추천 제품
ligand
dextrin
포장
pack of 5 × 1 mL
제조업체/상표
Cytiva 28-9187-78
저장 조건
(20% Ehtanol)
파라미터
42 psi
베드 크기
7 mm × 25 mm
베드 용적
1 mL
컬럼 I.D.
7 mm
기질
highly cross-linked 6% agarose
평균 부품 크기
34 μm
cleaning
2-13
working range
>7
용량
~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
저장 온도
2-8°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
MBPTrap™ HP columns prepacked with Dextrin Sepharose™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
특징 및 장점
- HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
- Physiological conditions and mild elution preserve the activity of the target protein.
- Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
기타 정보
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
법적 정보
MBPTrap is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
This page shows troubleshooting of methods for purification of MBP-tagged recombinant proteins using GE Healthcare products.
프로토콜
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.