추천 제품
ligand
recombinant protein G lacking albumin-binding region
포장
pkg of 50 × 100 μL
제조업체/상표
Cytiva 28-4083-47
저장 조건
(20% Ethanol)
베드 용적
100 μL
기질
highly cross-linked 6% agarose
평균 부품 크기
34 μm
cleaning
2-9
working range
3-9
용량
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(IgG/column)
저장 온도
2-8°C
관련 카테고리
일반 설명
Ab SpinTrap™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
애플리케이션
Ab SpinTrap™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.
The spin columns are also suitable for use in antibody screening experiments.
Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.
Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.
For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.
The spin columns are also suitable for use in antibody screening experiments.
Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.
Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.
For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.
특징 및 장점
- Rapid and convenient small-scale purification of IgG.
- Protein G Sepharose™ High Performance in prepacked spin columns.
- No filtration or centrifugation of different crude samples necessary for antibody purification.
- Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
- Elution conditions formatted for both LC-MS and electrophoresis analysis.
- Elution conditions formatted for both LC-MS and electrophoresis analysis.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
프로토콜
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.
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