추천 제품
ligand
glutathione
material
polypropylene and polyethylene
제조업체/상표
Cytiva 28-4055-00
포장
pkg of 4 x 96-well
크기
127.8 mm × 85.5 mm × 30.6 mm
Matrix
4% agarose
입자 크기
45-165 μm
평균 직경
90 μm
cleaning
4-13
작동 범위
4-13
용량
>25 mg binding capacity (horse liver GST/ml medium)
일반 설명
애플리케이션
Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
GST MultiTrap™ FF is prepacked with Glutathione Sepharose™ 4 Fast Flow, while GST MultiTrap™ 4B is prepacked with Glutathione Sepharose™ 4B.
특징 및 장점
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione Sepharose™ media for convenience.
- Easy and predictable scale-up using prepacked GSTrap™ FF, GSTPrep™ FF 16/10, and GSTrap™ 4B columns.
저장 및 안정성
분석 메모
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.
프로토콜
This page shows how to solubilize membrane proteins with products from GE Healthcare.
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
Characteristics of Glutathione Sepharose® Products
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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