GE28-4017-45

GSTrap^™ 4B

Cytiva 28-4017-45, pack of 5 × 1 mL

• UNSPSC 코드: 23151816
• NACRES: NA.56

속성

• ligand: glutathione
• 양식: prepacked column
• 교차: cross-linked
• 포장: pack of 5 × 1 mL
• 제조업체/상표: Cytiva 28-4017-45
• 저장 조건: (20% Ethanol)
• 파라미터: <4 mL/min flow rate; 42 psi (H₂O at 25°C)
• 기술: liquid chromatography (LC): suitable
• 베드 크기: 7 mm × 25 mm
• 베드 용적: 1 mL
• 컬럼 I.D.: 7 mm
• Matrix: 4% agarose
• 기질 활성군: glutathione functional group
• 입자 크기: 45-165 μm
• 평균 직경: 90 μm
• cleaning: 4-13
• 작동 범위: 4-13
• 용량: >25 mg binding capacity (horse liver GST/ml medium)
• 분리 기술: affinity

설명

일반 설명

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

애플리케이션

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

특징 및 장점

• High binding capacity.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
• GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

안전성 정보

• 픽토그램: GHS02
• 신호어: Warning
• 유해 및 위험 성명서: H226
• 예방조치 성명서: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids