추천 제품
양식
liquid
특징
wetted part: no
포장
pkg of 5 × 3 mL
제조업체/상표
Cytiva 17-6003-19
저장 조건
(Rehydration Solution -20 °C Reagent 2 - 8 °C)
색상
Colorless
배송 상태
dry ice
저장 온도
−20°C
관련 카테고리
일반 설명
DeStreak™ Rehydration Solution.
애플리케이션
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
특징 및 장점
- Improves reproducibility and quality of 2-D gels by preventing streaking.
- Eliminates extra spots caused by nonspecific oxidation of Proteins.
- Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
- Highly suitable for both analytical and preparative gels.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
DeStreak is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
12 - Non Combustible Liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page describes components of rehydration solutions for use in 2-D Electrophoresis with GE Healthcare products.
프로토콜
This page describes troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from GE Healthcare.
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