추천 제품
포장
pack of 100 mL
제조업체/상표
Cytiva 17-5318-02
Matrix
6% cross-linked agarose
입자 크기
45-165 μm
평균 직경
90 μm
cleaning in place
2-14(Ni2+-stripped medium.)
작동 범위
3-12(Ni2+-stripped medium.)
용량
~40 mg binding capacity(histidine-tagged protein)
적합성
suitable for bioprocess medium
일반 설명
Ni Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
Ni Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity..
Ni Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As A member of the BioProcess media range, Ni Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
특징 및 장점
- Designed for scaling up purification of histidine-tagged proteins
- High protein binding capacity and minimal leakage of Ni2? ions
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep™ and HisTrap™ column formats.
저장 및 안정성
분석 메모
법적 정보
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
How to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
프로토콜
This page shows how to purify histidine-tagged recombinant proteins manually with a syringe using HisTrap FF crude Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.