추천 제품
ligand
glutathione and 10-carbon linker arm
포장
pack of 500 mL
제조업체/상표
Cytiva 17-5132-03
Matrix
highly cross-linked 4% agarose
평균 직경
90 μm
cleaning
3-12
작동 범위
3-12
용량
>10 mg/mL binding capacity
관련 카테고리
일반 설명
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
특징 및 장점
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
저장 및 안정성
분석 메모
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
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문서
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.
프로토콜
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
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