추천 제품
ligand
glutathione and 10-carbon linker arm
포장
pack of 5 × 5 mL
제조업체/상표
Cytiva 17-5131-02
파라미터
flow rate
42 psi
베드 크기
16 mm × 25 mm
베드 용적
5 mL
컬럼 I.D.
16 mm
기질
highly cross-linked 4% agarose
평균 직경
90 μm
cleaning
3-12
working range
3-12
용량
>10 mg/mL binding capacity
관련 카테고리
일반 설명
GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.
* See the section for licensing information in our terms and conditions.
특징 및 장점
- Fast and simple one-step purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Easy scale-up by connecting the columns in series.
- Sepharose™ 4 Fast Flow provides good flow properties.
저장 및 안정성
분석 메모
기타 정보
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
프로토콜
This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
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