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Merck
모든 사진(1)

주요 문서

GE17-5068-01

Sigma-Aldrich

SOURCE™ 15RPC ST 4.6/100

pkg of 1 column

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모든 사진(1)

About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

ligand

matrix surface

material

polypropylene column

특징

autoclavable 20 min at 121°C

포장

pkg of 1 column

파라미터

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

기술

HPLC: suitable

베드 크기

4.6 mm × 100 mm

베드 용적

1.7 mL

컬럼 I.D.

4.6 mm

Matrix

polymeric
polystyrene/divinylbenzene

기질 활성군

polystyrene-divinylbenzene phase

평균 부품 크기

15 μm

cleaning

1-14

작동 범위

1-12

용량

~10 mg binding capacity

적합성

suitable for bioprocess medium

분리 기술

reversed phase

일반 설명

SOURCE™ 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

애플리케이션

SOURCE™ 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE™ 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE™ 15RPC offers properties superior to those of other polymeric matrices.

특징 및 장점

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE™ to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

프로토콜

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.