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GE17-3712-21

HIS Mag Sepharose™ Excel

Cytiva 17-3712-21, pack of 5 × 1 mL

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

ligand

IMAC immobilized with Ni

포장

pack of 5 × 1 mL

제조업체/상표

Cytiva 17-3712-21

파라미터

Ambient temp. range

기질

highly cross-linked spherical agarose including magnetite

관련 카테고리

일반 설명

His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.

Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.

The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.

Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.

특징 및 장점

  • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
  • Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
  • Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
  • Simplify handling using visible and dense Sepharose-based magnetic beads

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol, 5% medium slurry)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Environment
GHS09

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

문서

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

프로토콜

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from GE Healthcare.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.

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