추천 제품
ligand
IMAC immobilized with Ni
포장
pack of 2 × 1 mL
제조업체/상표
Cytiva 17-3712-20
파라미터
Ambient temp. range
기질
highly cross-linked spherical agarose including magnetite
일반 설명
His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.
Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.
The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.
Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.
Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.
Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.
The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.
Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.
Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.
특징 및 장점
- Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
- Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
- Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
- Simplify handling using visible and dense Sepharose-based magnetic beads
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol, 5% medium slurry)
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
프로토콜
This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from GE Healthcare.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.
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