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주요 문서

GE17-1194-01

Sigma-Aldrich

HiPrep 26/60 Sephacryl® S-100 HR

Cytiva 17-1194-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

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About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

material

polypropylene

분자량

10000-100000 Da (globular proteins)

포장

pkg of 1 ea

제조업체/상표

Cytiva 17-1194-01

파라미터

<13 mL sample volume
<2.6 mL/min flow rate
0.15 Mpa pressure (22 psi)

베드 용적

320 mL

컬럼 길이 × I.D.

60 cm × 26 mm

Matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

기질 활성군

dextran-acrylamide phase

평균 부품 크기

50 μm

cleaning

2-13

작동 범위

3-11

분리 기술

size exclusion (SEC)

일반 설명

HiPrep 26/60 Sephacryl® S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-100 is used for separating peptides and small proteins.

특징 및 장점

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offers reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

적합성

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 13 mL sample volume

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

프로토콜

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

This page shows how to perform a separation with a sephacryl column from GE Healthcare which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.

관련 콘텐츠

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

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