추천 제품
ligand
recombinant protein G lacking albumin-binding region
포장
pkg of 1 kit
제조업체/상표
Cytiva 17-1128-01
저장 조건
(20% Ethanol)
파라미터
<4 mL/min flow rate
42 psi
베드 크기
7 mm × 25 mm
베드 용적
1 mL
컬럼 I.D.
7 mm
Matrix
highly cross-linked 6% agarose
평균 부품 크기
34 μm
cleaning
2-9
작동 범위
3-9
용량
>1 mg binding capacity (lgG/column)
저장 온도
2-8°C
일반 설명
MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
애플리케이션
MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose™ High Performance.
특징 및 장점
- Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
- Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose™ High Performance and enough buffer concentrate for 20 preparations with a syringe.
- MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
기타 정보
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
법적 정보
HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
프로토콜
This page shows how to prepare samples for purification with affinity chromatography.
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