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Key Documents

GE17-1014-01

Q Sepharose High Performance

Cytiva 17-1014-01, pack of 75 mL

동의어(들):

Q Sepharose Resin

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

ligand

quaternary amine

설명

Ion Exchanger Type (value)

포장

pack of 75 mL

제조업체/상표

Cytiva 17-1014-01

기질

6% cross-linked agarose

입자 크기

24-44 μm

평균 부품 크기

34 μm

cleaning in place

1-14

working range

2-12

적합성

suitable for bioprocess medium

관련 카테고리

일반 설명

Q Sepharose High Performance is a strong anion exchange BioProcess resin with high resolution designed for high intermediate and polishing steps in downstream processing.

Q Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. Q Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide.

Q Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Q Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support.

애플리케이션

Ion Exchange Chromatography (IEX) separates molecules on the bases of differences in their net surface charge. Anion Exchange Chromatography (AIEX) medium interacts with negatively charged proteins and are separated by selective salt elution.

특징 및 장점

  • Strong anion exchanger for intermediate and final polishing steps
  • High resolution, high capacity separation with high recovery
  • High chemical stability for effective CIP/sanitization
  • BioProcess resin supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

문서

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

프로토콜

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

This page discusses various aspects of sample preparation for chromatographic purification.

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