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주요 문서

GE17-0998-01

Heparin Sepharose 6 Fast Flow

Cytiva 17-0998-01, pack of 50 mL

동의어(들):

Heparin Sepharose 6 Fast Flow

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

ligand

porcine heparin

포장

pack of 50 mL

제조업체/상표

Cytiva 17-0998-01

기질

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning in place

4-13

working range

4-12

적합성

suitable for bioprocess medium

일반 설명

Heparin Sepharose 6 Fast Flow is a well established BioProcess affinity medium designed for capture or intermediate purification steps. This medium is extensively used for industrial purification of several marketed plasma proteins.

Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.

This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.

Heparin Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Heparin Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

특징 및 장점

  • Industrial purification of antithrombin III, coagulation factors and other plasma proteins
  • Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
  • BioProcess medium supported for industrial applications and well-established in approved processes

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

문서

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

프로토콜

DNA binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the replication and orientation of the DNA such as histones, nucleosomes and replicases and those involved in transcription such as RNA/DNA polymerases, transcriptional activators and repressors and restriction enzymes.

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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