추천 제품
포장
pack of 100 mL
제조업체/상표
Cytiva 17-0921-08
Matrix
6% cross-linked agarose
입자 크기
45-165 μm
평균 직경
90 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
작동 범위
3-12
용량
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
적합성
suitable for bioprocess medium
일반 설명
IMAC Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
IMAC Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose™ 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.
IMAC Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
특징 및 장점
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in the convenient and time-saving prepacked HiTrap® and HiPrep™ formats.
저장 및 안정성
분석 메모
법적 정보
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
가장 최신 버전 중 하나를 선택하세요:
문서
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
How to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
프로토콜
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.