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Key Documents

GE17-0709-01

DEAE Sepharose Fast Flow

Cytiva 17-0709-01, pack of 500 mL

동의어(들):

DEAE Sepharose Resin

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

ligand

diethylaminoethyl

설명

Ion Exchanger Type (value)

포장

pack of 500 mL

제조업체/상표

Cytiva 17-0709-01

기질

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning in place

2-14

working range

2-12

적합성

suitable for bioprocess medium

일반 설명

DEAE Sepharose Fast Flow is a weak anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

DEAE Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. DEAE Sepharose Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with DEAE Sepharose Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, DEAE Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

특징 및 장점

  • Well-proven weak anion exchanger developed for industrial downstream processes
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

프로토콜

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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