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Merck
모든 사진(1)

주요 문서

G9293

Sigma-Aldrich

Anti-GAL4, Activation domain antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-Galactose 4

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.56

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

buffered aqueous solution

농도

~1.0 mg/mL

기술

western blot: 0.5 μg/mL using GAL4-AD fusion protein in Saccharomyces cerevisiae extract

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

GAL4 protein is an 881 amino acid transcription factor containing an independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a regulatory gene that belongs to GAL gene family. It is a component of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).
Useful in the study of protein:protein interaction studies. GAL4 DNA activation domain fusion proteins.

면역원

Synthetic peptide corresponding to amino acids of Saccharomyces cerevisiae GAL4 protein.

애플리케이션

Anti-GAL4, Activation domain antibody produced in rabbit has been used in western blotting.

생화학적/생리학적 작용

GAL4 induces the genes that are responsible for regulation of galactose metabolism in saccharomyces cerevisiae. GAL4 protein is widely used for studying transcriptional activation in eukaryotes. The yeast two hybrid system is widely used to study to discover protein− protein interactions (PPIs) and protein−DNA interactions.

물리적 형태

The product is provided as a solution of affinity isolated antibody in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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