G5671

Anti-Galectin-8 antibody, Mouse monoclonal

~1.0 mg/mL, clone VA-11.25, purified from hybridoma cell culture

• 동의어(들): ANTIGal-8, ANTILGALS8, ANTILectin, galectoside-binding, soluble 8, ANTIPCTA1, ANTIProstate carcinoma tumor antigen 1
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: VA-11.25, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~34 kDa
• 종 반응성: mouse, rat, human
• 농도: ~1.0 mg/mL
• 기술: immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using rat galectin 8 recombinant protein
• 동형: IgM
• UniProt 수납 번호: O00214
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... LGALS8(3964) ; mouse ... Lgals8(56048) ; rat ... Lgals8(116641)

설명

일반 설명

Galectins specifically bind to β-galactosides. 15 mammalian galectins have been identified so far. Galectin 8 contains two CRDs connected by a short linker peptide.

Monoclonal Anti-Galectin 8 (mouse IgM isotype) is derived from the hybridoma VA-11.25 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant rat galectin 8.Galectins belong to a family of carbohydrate-binding proteins. Within this family of proteins, galectin 8 is unique, as it exists in many forms encoded by the same gene.

특이성

The antibody has low cross-reactivity with galectin 3 and galectin 9.

면역원

recombinant rat galectin 8.

애플리케이션

Monoclonal Anti-galectin-8 antibody produced in mouse has been used for:

• immunoblotting
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)

생화학적/생리학적 작용

Galectins belong to a family of carbohydrate-binding proteins. These proteins share similarities in the carbohydrate recognition domain (CRD) and in their specificity for N-Acetyllactosamine-enriched glycoconjugates. The binding of galectins to saccharides modulates cell proliferation, cell death and cell migration, which are involved in cancer initiation and progression. Galectin 8 is a secreted protein that requires both of its CRDs to be active to modulate cell adhesion. It forms a complex with integrins and triggers integrin-mediated signaling cascades. On the contrary, excessive amounts of this protein negatively regulate cell adhesion by interacting with integrins. These signals assist in identifying regions available for cell adhesion and migration. It may be involved in neoplastic transformation.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)