G0282

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): GM-CSF
• MDL number: MFCD00241918
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Granulocyte-Macrophage Colony-Stimulating Factor from mouse, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 생물학적 소스: mouse
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥97% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: ≤0.2 ng/mL EC₅₀ (corresponds to ≥5 × 10⁶ units/mg)
• 품질: endotoxin tested
• 분자량: ~15 kDa (125 amino acids including N-terminal methionine)
• 포장: pkg of 5 and 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/mg
• 색상: white
• solubility: water: soluble, clear, colorless
• UniProt 수납 번호: P01587
• 저장 온도: −20°C
• 유전자 정보: mouse ... CSF2(12981) , Csf2(12981)

설명

생화학적/생리학적 작용

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

물리적 형태

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

분석 메모

The EC₅₀ activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)