F1180

Anti-Flotillin 1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-FLOT1, Anti-Reggie-2
• MDL number: MFCD01633353
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: ~48 kDa
• 종 반응성: rat, mouse, human
• 농도: ~1 mg/mL
• 기술: indirect immunofluorescence: 5-10 μg/mL using human HEK-293K cells; western blot: 0.5-1 μg/mL using whole extract of mouse lung; western blot: 1-2 μg/mL using whole extract of rat lung
• UniProt 수납 번호: O75955
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FLOT1(10211) ; mouse ... Flot1(14251) ; rat ... Flot1(64665)

설명

일반 설명

Flotillin 1(FLOT1), also known as reggie-2, is an estrogen responsive gene mapped to human chromosome 6p21.33. This gene codes for an integral membrane protein and a component of lipid rafts.

Flotillins are lipid raft associated proteins expressed mainly in adipose tissue, striated muscle tissues, and lung. It has a crucial role in several cellular processes like phagocytosis, endocytosis, insulin signalling and neuronal regeneration. Anti-flotillin 1 antibody can be used in immunobloting and immunolocalization. Rabbit anti- flotillin 1 antibody reacts specifically with flotillin 1 of rat, mouse and human.

면역원

synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.

애플리케이션

Anti-flotillin 1 antibody can be used in immunofluorescence and western blotting.

Anti-flotillin 1 antibody produced in rabbit has been used in immunoblot analysis.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

생화학적/생리학적 작용

Flotillin 1 (FLOT1) has a role in endocytosis. It also functions in growth factor signaling and activates mitogen-activated protein kinase signaling. In addition, it is also involved in phagocytosis, regulation of actin cytoskeleton and membrane trafficking. The protein has been associated with tumor progression and is expressed in many cancers, including breast cancer.

표적 설명

Flotillin 1 encodes a caveolae-associated, integral membrane protein. Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)