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Merck
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주요 문서

EC600

Sigma-Aldrich

GenElute-E Single Spin DNA Cleanup Kit

greener alternative

동의어(들):

E Single Spin DNA Cleanup Kit, GenElute DNA Cleanup Kit

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About This Item

UNSPSC 코드:
41105600
NACRES:
NA.52

정제법

(Single-spin negative chromotography)
(Time: 3 minutes or less)

특징

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

기술

DNA purification: suitable

환경친화적 대안 카테고리

저장 온도

room temp

일반 설명

The GenElute-E Single Spin DNA Cleanup Kit is a one-step cleanup kit for the depletion of impurities and partial fractions (<50 bp) from pre-purified samples of DNA. The kit efficiently removes salt, detergent, nucleotides, primers, proteins/enzymes, organic solvents and many other inhibitors of downstream applications. The standard protocol, which can be completed in 3 minutes or less, is suitable for DNA fragments, plasmid DNA, and genomic DNA.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

애플리케이션

Traditional methods for purifying genomic DNA rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute-E Single Spin DNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, and dyes from DNA solutions. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.

특징 및 장점

Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

저장 및 안정성

Use within 12 months of receipt.

기타 정보

We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised.  GenElute-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.

법적 정보

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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관련 콘텐츠

Answers to frequently asked questions related to GenElute™-E single spin DNA and RNA purification and negative chromatography

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