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Merck
모든 사진(1)

Key Documents

E8156

Sigma-Aldrich

Anti-eRF1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-Eukaryotic translation termination factor 1, Anti-RF1, Anti-Release factor 1, Anti-eTF1

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

분자량

antigen 50 kDa

종 반응성

rat, human, mouse

포장

antibody small pack of 25 μL

농도

~1 mg/mL

기술

immunoprecipitation (IP): 1-2 μg using HeLa cell lysates.
western blot: 0.5-1 μg/mL using 3T3 or Rat1 cell extracts.

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... ETF1(2107)
mouse ... Etf1(225363)
rat ... Etf1(307503)

일반 설명

Eukaryotic translation termination factor 1 (eRF1) is a protein encoded by the ETF1 gene in humans. It belongs to eRF1 family and the amino-acid sequence is highly conserved.

애플리케이션

Anti-eRF1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

생화학적/생리학적 작용

Eukaryotic translation termination factor 1 (eRF1) proteins are directly implicated in the termination of translation in eukaryotes. It plays an important role in the termination of protein synthesis, which is performed by three domains (N, M and C) of eRF1. Rearrangement in eRF1 leads to N-domain accommodation into the ribosomal A site.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Identification and characterization of a novel ubiquitous nucleolar protein `NARR?encoded by a gene overlapping the rab34 oncogene
Zougman A, et al.
Nucleic Acids Research, 39(16), 7103-7113 (2011)
Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division
Petsch K, et al.
Plant Physiology, 139(1), 115-126 (2005)
Leo E Wong et al.
Nucleic acids research, 40(12), 5751-5765 (2012-03-03)
Translation termination in eukaryotes is catalyzed by two release factors eRF1 and eRF3 in a cooperative manner. The precise mechanism of stop codon discrimination by eRF1 remains obscure, hindering drug development targeting aberrations at translation termination. By solving the solution
Polina Kryuchkova et al.
Nucleic acids research, 41(8), 4573-4586 (2013-02-26)
Release factor eRF1 plays a key role in the termination of protein synthesis in eukaryotes. The eRF1 consists of three domains (N, M and C) that perform unique roles in termination. Previous studies of eRF1 point mutants and standard/variant code
Alexandre Zougman et al.
Nucleic acids research, 39(16), 7103-7113 (2011-05-19)
There are only few reports on protein products originating from overlapping mammalian genes even though computational predictions suggest that an appreciable fraction of mammalian genes could potentially overlap. Mass spectrometry-based proteomics has now acquired the tools to probe proteins in

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