E5389

Erythromycin

BioReagent, suitable for cell culture

• 동의어(들): E-Mycin, Erythrocin
• 실험식(Hill 표기법): C₃₇H₆₇NO₁₃
• CAS Number: 114-07-8
• Molecular Weight: 733.93
• Beilstein: 75279
• EC Number: 204-040-1
• MDL number: MFCD00084654
• UNSPSC 코드: 12352207
• PubChem Substance ID: 24894612
• NACRES: NA.76

속성

• 제품 라인: BioReagent
• Quality Level: 200
• 형태: powder
• 효능: ≥850 μg per mg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤0.1 EU/mg endotoxin
• 색상: white
• mp: 133 °C
• solubility: 2 M HCl: 50 mg/mL (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.); ethanol: soluble (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
• 항생제 활성 스펙트럼: Gram-negative bacteria; Gram-positive bacteria
• 동작 모드: protein synthesis | interferes
• SMILES string: CC[C@H]1OC(=O)[C@H](C)[C@@H](O[C@H]2C[C@@](C)(OC)[C@@H](O)[C@H](C)O2)[C@H](C)[C@@H](O[C@@H]3O[C@H](C)C[C@@H]([C@H]3O)N(C)C)[C@](C)(O)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@]1(C)O
• InChI: 1S/C37H67NO13/c1-14-25-37(10,45)30(41)20(4)27(39)18(2)16-35(8,44)32(51-34-28(40)24(38(11)12)15-19(3)47-34)21(5)29(22(6)33(43)49-25)50-26-17-36(9,46-13)31(42)23(7)48-26/h18-26,28-32,34,40-42,44-45H,14-17H2,1-13H3/t18-,19-,20+,21+,22-,23+,24+,25-,26+,28-,29+,30-,31+,32-,34+,35-,36-,37-/m1/s1
• InChI key: ULGZDMOVFRHVEP-RWJQBGPGSA-N
• 유전자 정보: human ... ABCB1(5243) , CYP3A4(1576) , MLNR(2862) ; mouse ... Abcb1a(18671) , Abcb1b(18669)

설명

일반 설명

Chemical structure: macrolide

Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains.

애플리케이션

Erythromycin has been used:

• as a supplement for nutrient broth medium for culturing green fluorescent protein (GFP)- expressing E. coli
• as a model drug to determine small intestinal (SMI) microtissue viability using the MTT assay{254
• as an antibiotic to study the treatment strategies of chronic infections

생화학적/생리학적 작용

Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.

Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria.

주의사항

This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.

제조 메모

This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)