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Merck
모든 사진(4)

Key Documents

E2760

Sigma-Aldrich

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone 29.1, ascites fluid

동의어(들):

Anti-ERBB, Anti-ERBB1, Anti-ERRP, Anti-HER1, Anti-NISBD2, Anti-PIG61, Anti-mENA

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

29.1, monoclonal

포함

15 mM sodium azide

종 반응성

pig, human, rat, mouse, canine

반응하면 안 됨

rabbit

기술

indirect ELISA: 1:10,000 using extract of the A431 cell line
western blot: 1:3,000 using extract of the A431 cell line

동형

IgG1

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)

일반 설명

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. A close similarity has been found between the sequence of the avian erythroblastic leukemia viral oncogene homolog (v-erb-B) and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R).

특이성

Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.

면역원

human carcinoma cell line A431, which expresses high levels of EGF receptors.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
May be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis.
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunoaffinity
  • immunohistochemistry
  • western blotting

생화학적/생리학적 작용

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (Neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

B Assarsson et al.
Acta physiologica Scandinavica, 154(2), 177-183 (1995-06-01)
Members of the epidermal growth factor (EGF) family have many different effects on ovarian cells including a strong inhibition of estradiol-17 beta production. An increased binding of the EGF family to its receptor is seen at the time of ovulation.
Nanoscale imaging of epidermal growth factor receptor clustering effects of inhibitors
Abulrob A, et al.
The Journal of biological chemistry, 285(5), 3145-3156 (2010)
Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer
Mitsudomi T and Yatabe Y
The FEBS journal, 277(2), 301-308 (2010)
David J Javier et al.
Journal of nanophotonics, 2(1), 23506-23506 (2008-12-11)
Anisotropic metal-based nanomaterials have been proposed as potential contrast agents due to their strong surface plasmon resonance. We evaluated the contrast properties of gold, silver, and gold-silver hybrid nanorods for molecular imaging applications in three-dimensional biological samples. We used diffuse
Targeting the EGFR signaling pathway in cancer therapy
Seshacharyulu P et al
Expert Opinion on Therapeutic Targets, 16, 15-31 (2010)

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