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D6442

Sigma-Aldrich

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR

Ready-to-use 2x mix for qPCR with ROX

동의어(들):

Taq polymerase mix

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About This Item

UNSPSC 코드:
41106300
NACRES:
NA.55

형태

liquid

사용

sufficient for 20 reactions
 sufficient for 2000 reactions
 sufficient for 400 reactions

특징

dNTPs included
hotstart

농도

2.5 units/reaction (50 μL reaction volume)

기술

qPCR: suitable

색상

colorless

입력

purified DNA

응용 분야

agriculture

호환성

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

검출 방법

probe-based

배송 상태

wet ice

저장 온도

−20°C

일반 설명

JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.
To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired.

애플리케이션

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used:
  • For quantitative polymerase chain reaction (qPCR) of cDNA template
  • As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes
  • For qPCR amplifications using SYBR
  • For multiplex PCR
  • For reduction of primer dimers

특징 및 장점

  • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • When performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time and the risk of contamination from multiple pipetting steps
  • This master mix allows consistent batch-to-batch and reaction-to-reaction performance
  • Internal Reference Dye is provided for reaction normalization

포장

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

수량

Use 2.5 units per reaction.

법적 정보

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

관련 제품

제품 번호
설명
가격

W1754

Water, PCR Reagent

픽토그램

Flame over circleCorrosionExclamation mark
GHS03,GHS05,GHS07

신호어

Danger

유해 및 위험 성명서

H272,H315,H317,H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Ox. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

5.1B - Oxidizing hazardous materials

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Evrim Komurcu-Bayrak et al.
Clinica chimica acta; international journal of clinical chemistry, 383(1-2), 110-115 (2007-06-09)
We evaluated the relationship of the lipoprotein lipase (LPL) S447X variant with serum lipid levels and the metabolic syndrome (MS) in the Turkish Adult Risk Factor (TARF) study. This is the first study examining this LPL variant in the Turkish
Irene B Hanning et al.
Methods in molecular biology (Clifton, N.J.), 733, 159-170 (2011-03-25)
Next-generation sequencing (NGS) is a powerful tool that can be utilized to profile and compare microbial populations. By amplifying a target gene present in all bacteria and subsequently sequencing amplicons, the bacteria genera present in the populations can be identified
Edouard Jurkevitch
Current protocols in microbiology, Chapter 7, Unit7B-Unit7B (2012-08-10)
Bdellovibrio and like organisms (BALOs) are obligate predators of Gram-negative bacteria. BALOs are isolated as plaques growing at the expense of their prey and are cultivated as two-member cultures. The growth cycle is composed of an extracellular attack phase and
Adrien Chabot et al.
Genetics, 176(4), 2069-2076 (2007-06-15)
Most phenotypic differences between human and chimpanzee are likely to result from differences in gene regulation, rather than changes to protein-coding regions. To date, however, only a handful of human-chimpanzee nucleotide differences leading to changes in gene regulation have been
Bilge Ozsait et al.
Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, 8(5), 324-330 (2008-10-14)
In this study, our aim was to investigate the association of cholesterol ester transfer protein (CETP) TaqIB polymorphism with the likelihood of metabolic syndrome (MetS). Study was designed as a cross-sectional analysis of the Turkish Adult Risk Factor follow-up study.
모든 관련된 서류 보기

문서

Probe-based qPCR

Probe based QPCR utilizes a fluorescent–labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.

Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

관련 콘텐츠

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

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