D1194

Anti-Derlin-2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-DERL2, Anti-Der1-like domain family, member 2
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~21 kDa
• 종 반응성: human, mouse
• 농도: ~1.0 mg/mL
• 기술: indirect immunofluorescence: 5-10 μg/mL using HeLa cells; western blot: 2.0-4.0 μg/mL using whole extract of mouse 3T3 cells
• UniProt 수납 번호: Q9GZP9
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... DERL2(51009) ; mouse ... Derl2(116891)

설명

일반 설명

Derlin-1, Derlin-2, and Derlin-3 are the mammalian homologues of yeast Der1p, a transmembrane protein required for yeast endoplasmic reticulum-associated degradation (ERAD). Derlin-2 is approximately 30% identical to Derlin-1. In rat Derlin-2 is present to the endoplasmic reticulum(ER) membrane and forms a multisubunit complex with other proteins.

면역원

synthetic peptide corresponding to amino acid residues 223-239 of human derlin-2 conjugated to KLH via an N-terminal added cysteine residue. The corresponding sequence is identical in mouse.

애플리케이션

Anti-Derlin-2 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

생화학적/생리학적 작용

Derlin-2, also known as F-LANa, is involved in the degradation of misfolded glycoproteins in the ER. Derlin-2 shares ~30% sequence identity with Derlin-1 and spans the lipid bilayer of the ER four times, showing structural similarity to Derlin-1. It is a component of the mammalian ER-associated degradation (ERAD) mechanism and is upregulated by unfolded protein response (UPR). Overexpression of this gene leads to increase in degradation of misfolded glycoprotein, whereas its knockdown blocks degradation. Derlin-2 also interacts with the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)