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Merck
모든 사진(5)

주요 문서

CMC0022

Sigma-Aldrich

OverExpress™ C43(DE3) Electrocompetent Cells

for the highest protein expression

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모든 사진(5)

About This Item

UNSPSC 코드:
12352200
NACRES:
NA.85

생물학적 소스

Escherichia coli

Grade

for molecular biology

성장 모드

adherent or suspension

형태학

rod shaped

기술

microbiological culture: suitable

세포 형질전환

competent cell type: electrocompetent
transformation efficiency: ≥1 x 109 cfu/μg

배송 상태

dry ice

저장 온도

−70°C

일반 설명

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

특징 및 장점

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

성분

  • OverExpress C43(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

법적 정보

OverExpress is a trademark of Imaxio, S.A.

Storage Class Code

10 - Combustible liquids

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시험 성적서(COA)

Lot/Batch Number
SLCH5500
SLCF7490
SLCC1514
SLBX8788
SLBT3790

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COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

A Rangunwala et al.
Epidemiology and infection, 142(10), 2147-2154 (2013-12-18)
Crimean-Congo haemorrhagic fever virus (CCHFV) has the propensity to cause nosocomial infections with a high fatality rate. Handling the virus requires biosafety level-4 facilities, limiting accessibility for many laboratories. Advances in molecular techniques have allowed preparation of safe recombinant antigens
Arthur Laganowsky et al.
Nature, 510(7503), 172-175 (2014-06-06)
Previous studies have established that the folding, structure and function of membrane proteins are influenced by their lipid environments and that lipids can bind to specific sites, for example, in potassium channels. Fundamental questions remain however regarding the extent of

프로토콜

OverExpress™ Electrocompetent Cells

OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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